Part:BBa_K5377800:Design
RF self-induced production system
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1892
Illegal PstI site found at 3143 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 1892
Illegal PstI site found at 3143 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4220
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1892
Illegal PstI site found at 3143 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1892
Illegal PstI site found at 3143
Illegal AgeI site found at 1739
Illegal AgeI site found at 3563
Illegal AgeI site found at 3973 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. The ribB FMN riboswitch is from the Escherichia coli. If you want to use it in other strains, please ensure that the riboswitch RBS sequence can be recognized.
2. The titer of RF has only been validated in Escherichia coli.
Source
ribB FMN riboswitch is from E. coli.
ribA, ribB, ribD, ribE, ribC are from E. coli.
The pHG101 plasmid (contain J23106 promoter) is synthesized by RuiBiotech Co., Ltd.
References
[1]Serganov, A., Huang, L., & Patel, D. J. (2009). Coenzyme recognition and gene regulation by a flavin mononucleotide riboswitch. Nature, 458(7235), 233–237. https://doi.org/10.1038/nature07642
[2]Winkler, W. C., Cohen-Chalamish, S., & Breaker, R. R. (2002). An mRNA structure that controls gene expression by binding FMN. Proceedings of the National Academy of Sciences of the United States of America, 99(25), 15908-15913. https://doi.org/10.1073/pnas.212628899
[3] de Boer, H. A., Comstock, L. J., & Vasser, M. (1983). The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academy of Sciences of the United States of America, 80(1), 21–25. https://doi.org/10.1073/pnas.80.1.21
[4] JACOB, F., & MONOD, J. (1961). Genetic regulatory mechanisms in the synthesis of proteins. Journal of molecular biology, 3, 318–356. https://doi.org/10.1016/s0022-2836(61)80072-7
[5] Müller-Hill, B. (1996). The lac Operon: A Short History of a Genetic Paradigm. Berlin, New York: De Gruyter. https://doi.org/10.1515/9783110879476